MRNA-Driven Generation of Transgene-Free Neural Stem Cells from Human Urine-Derived Cells.

ABSTRACT: Human neural stem cells (NSCs) hold enormous promise for neurological disorders, typically requiring their expandable and differentiable properties for regeneration of damaged neural tissues. Despite the therapeutic potential of induced NSCs (iNSCs), a major challenge for clinical feasibility is the presence of integrated transgenes in the host genome, contributing to the risk for undesired genotoxicity and tumorigenesis. Here, we describe the advanced transgene-free generation of iNSCs from human urine-derived cells (HUCs) by combining a cocktail of defined small molecules with self-replicable mRNA delivery. The established iNSCs were completely transgene-free in their cytosol and genome and further resembled human embryonic stem cell-derived NSCs in the morphology, biological characteris

SUBMITTER: Kang PJ 

PROVIDER: S-EPMC6769943 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature