Viral Metagenomics in the Clinical Realm: Lessons Learned from a Swiss-Wide Ring Trial.

ABSTRACT: Shotgun metagenomics using next generation sequencing (NGS) is a promising technique to analyze both DNA and RNA microbial material from patient samples. Mostly used in a research setting, it is now increasingly being used in the clinical realm as well, notably to support diagnosis of viral infections, thereby calling for quality control and the implementation of ring trials (RT) to benchmark pipelines and ensure comparable results. The Swiss NGS clinical virology community therefore decided to conduct a RT in 2018, in order to benchmark current metagenomic workflows used at Swiss clinical virology laboratories, and thereby contribute to the definition of common best practices. The RT consisted of two parts (increments), in order to disentangle the variability arising from the experimental

SUBMITTER: Junier T 

PROVIDER: S-EPMC6770386 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature