Influenza A Hemagglutinin Passage Bias Sites and Host Specificity Mutations.

ABSTRACT: Animal studies aimed at understanding influenza virus mutations that change host specificity to adapt to replication in mammalian hosts are necessarily limited in sample numbers due to high cost and safety requirements. As a safe, higher-throughput alternative, we explore the possibility of using readily available passage bias data obtained mostly from seasonal H1 and H3 influenza strains that were differentially grown in mammalian (MDCK) and avian cells (eggs). Using a statistical approach over 80,000 influenza hemagglutinin sequences with passage information, we found that passage bias sites are most commonly found in three regions: (i) the globular head domain around the receptor binding site, (ii) the region that undergoes pH-dependent structural changes and (iii) the unstructured N

SUBMITTER: Lee RTC 

PROVIDER: S-EPMC6770435 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature