Detection and identification of allergens from Canadian mustard varieties of Sinapis alba and Brassica juncea.

ABSTRACT: Currently, information on the allergens profiles of different mustard varieties is rather scarce. Therefore, the objective of this study was to assess protein profiles and immunoglobulin E (IgE)-binding patterns of selected Canadian mustard varieties. Optimization of a non-denaturing protein extraction from the seeds of selected mustard varieties was first undertaken, and the various extracts were quantitatively and qualitatively analyzed by means of protein recovery determination and protein profiling. The IgE-binding patterns of selected mustard seeds extracts were assessed by immunoblotting using sera from mustard sensitized and allergic individuals. In addition to the known mustard allergens-Sin a 2 (11S globulins), Sin a 1, and Bra j 1 (2S albumins)-the presence of other new IgE-bindi

SUBMITTER: L'Hocine L 

PROVIDER: S-EPMC6770452 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature