Genotyping of the EIF1AY Gene in Iranian Patients with Non-Obstructive Azoospermia.

ABSTRACT:

Background

EIF1AY is one of the genes essential for normal spermatogenesis and is located in azoospermic factors region.

Objective

The present study was designed to investigate the EIF1AY gene nucleotide variations, and correlate it with spermatogenic maturation arrest and azoospermia in Iranian population.

Methods

A total number of 30 Iranian idiopathic non-obstructive azoospermic patients were selected as case group and 30 fertile men served as a control group who had at least 1 child. Nucleotide variation was analyzed in exon 3 and exon 5 in EIF1AY gene of both groups. DNA extraction from peripheral blood samples of selected individuals was done followed by amplification by PCR and sequencing with Sangar method.

Results

Totally 3 single nucleotide variations were identified: one in the intronic region of exon 3, next one in non-coding transcript exon variant (rs13447352) and the third one in the exonic region of exon 5, all were registered in NCBI-Gene database.

Conclusion

There was no statistically significant difference in the incidence of nucleotide variation between 2 study populations (p > 0.05). Further studies are required to specify the effects of Y: T20588295G variation on modification of protein structure, as well as the expression pattern of the gene and its association with azoospermia.

SUBMITTER: Yarahmadi E 

PROVIDER: S-EPMC6771061 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature