An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion.

ABSTRACT: Rapid perturbation of protein function permits the ability to define primary molecular responses while avoiding downstream cumulative effects of protein dysregulation. The auxin-inducible degron (AID) system was developed as a tool to achieve rapid and inducible protein degradation in nonplant systems. However, tagging proteins at their endogenous loci results in chronic auxin-independent degradation by the proteasome. To correct this deficiency, we expressed the auxin response transcription factor (ARF) in an improved inducible degron system. ARF is absent from previously engineered AID systems but is a critical component of native auxin signaling. In plants, ARF directly interacts with AID in the absence of auxin, and we found that expression of the ARF PB1 (Phox and Bem1) domain suppres

SUBMITTER: Sathyan KM 

PROVIDER: S-EPMC6771385 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature