The strand-biased mitochondrial DNA methylome and its regulation by DNMT3A.

ABSTRACT: How individual genes are regulated from a mitochondrial polycistronic transcript to have variable expression remains an enigma. Here, through bisulfite sequencing and strand-specific mapping, we show mitochondrial genomes in humans and other animals are strongly biased to light (L)-strand non-CpG methylation with conserved peak loci preferentially located at gene-gene boundaries, which was also independently validated by MeDIP and FspEI digestion. Such mtDNA methylation patterns are conserved across different species and developmental stages but display dynamic local or global changes during development and aging. Knockout of DNMT3A alone perturbed mtDNA regional methylation patterns, but not global levels, and altered mitochondrial gene expression, copy number, and oxygen respirati

SUBMITTER: Dou X 

PROVIDER: S-EPMC6771398 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature