Identification of proteins in the postsynaptic density fraction by mass spectrometry.

ABSTRACT: Our understanding of the organization of postsynaptic signaling systems at excitatory synapses has been aided by the identification of proteins in the postsynaptic density (PSD) fraction, a subcellular fraction enriched in structures with the morphology of PSDs. In this study, we have completed the identification of most major proteins in the PSD fraction with the use of an analytical method based on mass spectrometry coupled with searching of the protein sequence databases. At least one protein in each of 26 prominent protein bands from the PSD fraction has now been identified. We found 7 proteins not previously known to be constituents of the PSD fraction and 24 that had previously been associated with the PSD by other methods. The newly identified proteins include the heavy chain of myo

SUBMITTER: Walikonis RS 

PROVIDER: S-EPMC6772646 | biostudies-literature | 2000 Jun

REPOSITORIES: biostudies-literature