Activation of YAP attenuates hepatic damage and fibrosis in liver ischemia-reperfusion injury.

ABSTRACT:

Background & aims

Hepatic ischemia-reperfusion injury (IRI) is a major complication of hemorrhagic shock, liver resection and transplantation. YAP, a key downstream effector of the Hippo pathway, is essential for determining cell fate and maintaining homeostasis in the liver. We aimed to elucidate its role in IRI.

Methods

The role of YAP/Hippo signaling was systematically studied in biopsy specimens from 60 patients after orthotopic liver transplantation (OLT), and in a mouse model of liver warm IRI. Human biopsy specimens were collected after 2-10 h of cold storage and 3 h post-reperfusion, before being screened by western blot. In the mouse model, the role of YAP was probed by activating or inhibiting YAP prior to ischemia-reperfusion.

Results

In human biopsies, hig

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6773499 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature