Genetically Encoded, Photostable Indicators to Image Dynamic Zn²⁺ Secretion of Pancreatic Islets.

ABSTRACT: As an essential element for living organisms, zinc (Zn²⁺) exerts its biological functions both intracellularly and extracellularly. Previous studies have reported a number of genetically encoded Zn²⁺ indicators (GEZIs), which have been widely used to monitor Zn²⁺ in the cytosol and intracellular organelles. However, it is challenging to localize existing GEZIs to the extracellular space to detect secreted Zn²⁺. Herein, we report two photostable, green fluorescent protein (GFP) based indicators, ZIBG1 and ZIBG2, which respond to Zn²⁺ selectively and have affinities suited for detecting Zn²⁺ secretion from intracellular vesicles. In particular, ZIBG2 can be effectively targeted to the extracellular side of plasma membrane. We

SUBMITTER: Chen M 

PROVIDER: S-EPMC6773511 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature