Persistent effects on bovine granulosa cell transcriptome after resolution of uterine disease.

ABSTRACT: Metritis is associated with reduced fertility in dairy cows, but the mechanisms are unclear because the disease resolves several weeks before insemination. One hypothesis is that metritis causes persistent changes in granulosa cells during follicle development, which might be evident in the transcriptome of granulosa cells from dominant follicles weeks after parturition. To test this hypothesis, we collected the follicular fluid and granulosa cells from dominant follicles 63 days post partum from cows previously diagnosed with metritis, at least 6 weeks after resolution of the disease and from cows not diagnosed with metritis (control cows). Bacterial lipopolysaccharide was detected in follicular fluid, and concentrations were associated with follicular fluid IL-8 and glucose concentration

SUBMITTER: Piersanti RL 

PROVIDER: S-EPMC6773536 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature