Multiplex isolation and profiling of extracellular vesicles using a microfluidic DICE device.

ABSTRACT: Profiling of extracellular vesicles (EVs) is an emerging area in the field of liquid biopsies because of their innate significance in diseases and abundant information reflecting disease status. However, unbiased enrichment of EVs and thorough profiling of EVs is challenging. In this paper, we present a simple strategy to immobilize and analyze EVs for multiple markers on a single microfluidic device and perform differentiated immunostaining-based characterization of extracellular vesicles (DICE). This device, composed of four quadrants with a single inlet, captures biotinylated EVs efficiently and facilitates multiplexed immunostaining to profile their extracellular proteins, allowing for a multiplexed approach for non-invasive cancer diagnostics in the future. From controlled sample expe

SUBMITTER: Kang YT 

PROVIDER: S-EPMC6774196 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature