Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.

ABSTRACT: Membrane proteins mediate a number of cellular functions and are associated with several diseases and also play a crucial role in pathogenicity. Due to their importance in cellular structure and function, they are important drug targets for ~60% of drugs available in the market. Despite the technological advancement and recent successful outcomes in determining the high-resolution structural snapshot of membrane proteins, the mechanistic details underlining the complex functionalities of membrane proteins is least understood. This is largely due to lack of structural dynamics information pertaining to different functional states of membrane proteins in a membrane environment. Fluorescence spectroscopy is a widely used technique in the analysis of functionally-relevant structure and dynamic

SUBMITTER: Raghuraman H 

PROVIDER: S-EPMC6774292 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature