Application of metagenomic shotgun sequencing to detect vector-borne pathogens in clinical blood samples.

ABSTRACT:

Background

Vector-borne pathogens are a significant public health concern worldwide. Infections with these pathogens, some of which are emerging, are likely under-recognized due to the lack of widely-available laboratory tests. There is an urgent need for further advancement in diagnostic modalities to detect new and known vector-borne pathogens. We evaluated the utility of metagenomic shotgun sequencing (MGS) as a pathogen agnostic approach for detecting vector-borne pathogens from human blood samples.

Methods

Residual whole blood samples from patients with known infection with Babesia microti, Borrelia hermsii, Plasmodium falciparum, Mansonella perstans, Anaplasma phagocytophilum or Ehrlichia chaffeensis were studied. Samples underwent DNA extraction, removal of human DNA,

SUBMITTER: Vijayvargiya P 

PROVIDER: S-EPMC6774502 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature