Genome architecture and stability in the Saccharomyces cerevisiae knockout collection.

ABSTRACT: Despite major progress in defining the functional roles of genes, a complete understanding of their influences is far from being realized, even in relatively simple organisms. A major milestone in this direction arose via the completion of the yeast Saccharomyces cerevisiae gene-knockout collection (YKOC), which has enabled high-throughput reverse genetics, phenotypic screenings and analyses of synthetic-genetic interactions^1-3. Ensuing experimental work has also highlighted some inconsistencies and mistakes in the YKOC, or genome instability events that rebalance the effects of specific knockouts^4-6, but a complete overview of these is lacking. The identification and analysis of genes that are required for maintaining genomic stability have traditionally relied on re

SUBMITTER: Puddu F 

PROVIDER: S-EPMC6774800 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature