Saliva-derived cfDNA is applicable for EGFR mutation detection but not for quantitation analysis in non-small cell lung cancer.

ABSTRACT:

Background

Both quantitative and qualitative aspects of plasma cell-free DNA (plasma cfDNA, pcfDNA) have been well-studied as potential biomarkers in non-small cell lung cancer (NSCLC). Accumulating evidence has proven that saliva also has the potential for the detection and analysis of circulating free DNA (saliva cfDNA, scfDNA).

Methods

In the current study, we aimed to explore the potential application of scfDNA in NSCLC diagnostics and consistency of epidermal growth factor receptor (EGFR) mutation detection in paired pcfDNA and scfDNA using droplet digital PCR (ddPCR) and analyze the relationship between EGFR mutations and clinical treatment response.

Results

In the quantitative cohort study, scfDNA concentration in NSCLC patients was no different from that in he

SUBMITTER: Ding S 

PROVIDER: S-EPMC6775000 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature