Development of experimental silicosis in inbred and outbred mice depends on instillation volume.

ABSTRACT: There is considerable variation in methods to induce experimental silicosis with the effects of dose and route of exposure being well documented. However, to what extent the volume of silica suspension alters the dispersion and severity of silicosis has not been adequately investigated. In this study, the optimal volume of a crystalline silica suspension required to obtain uniform distribution and greatest incidence and severity of silicosis was determined in inbred and outbred mice. Silica dispersal, detected by co-inspiration with India ink and polarized light microscopy, was highly dependent upon volume. Furthermore, although peribronchitis, perivasculitis, and increases in bronchoalveolar lavage fluid cell numbers were detected a lower doses and volumes, significant alveolitis required

SUBMITTER: Mayeux JM 

PROVIDER: S-EPMC6775097 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature