Enrichment of hematopoietic stem/progenitor cells in the zebrafish kidney.

ABSTRACT: Hematopoietic stem cells (HSCs) maintain the entire blood system throughout life and are utilized in therapeutic approaches for blood diseases. Prospective isolation of highly purified HSCs is crucial to understand the molecular mechanisms underlying regulation of HSCs. The zebrafish is an elegant genetic model for the study of hematopoiesis due to its many unique advantages. It has not yet been possible, however, to purify HSCs in adult zebrafish due to a lack of specific HSC markers. Here we show the enrichment of zebrafish HSCs by a combination of two HSC-related transgenes, gata2a:GFP and runx1:mCherry. The double-positive fraction of gata2a:GFP and runx1:mCherry (gata2a⁺ runx1⁺) was detected at approximately 0.16% in the kidney, the main hematopoietic organ in te

SUBMITTER: Kobayashi I 

PROVIDER: S-EPMC6775131 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature