DNA recovery from archived RDTs for genetic characterization of Plasmodium falciparum in a routine setting in Lambarene, Gabon.

ABSTRACT:

Background

Rapid diagnostic tests (RDTs) have been described as a source of genetic material to analyse malaria parasites in proof-of-concept studies. The increasing use of RDTs (e.g., in focal or mass screening and treatment campaigns) makes this approach particularly attractive for large-scale investigations of parasite populations. In this study, the complexity of Plasmodium falciparum infections, parasite load and chloroquine resistance transporter gene mutations were investigated in DNA samples extracted from positive RDTs, obtained in a routine setting and archived at ambient temperature.

Methods

A total of 669 archived RDTs collected from malaria cases in urban, semi-urban and rural areas of central Gabon were used for P. falciparum DNA extraction. Performance of RDTs

SUBMITTER: Nguyen TT 

PROVIDER: S-EPMC6775649 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature