HutZ is required for biofilm formation and contributes to the pathogenicity of Edwardsiella piscicida.

ABSTRACT: Edwardsiella piscicida is a severe fish pathogen. Haem utilization systems play an important role in bacterial adversity adaptation and pathogenicity. In this study, a speculative haem utilization protein, HutZ_Ep, was characterized in E. piscicida. hutZ_Ep is encoded with two other genes, hutW and hutX, in an operon that is similar to the haem utilization operon hutWXZ identified in V. cholerae. However, protein activity analysis showed that HutZ_Ep is probably not related to hemin utilization. To explore the biological role of HutZ_Ep, a markerless hutZ_Ep in-frame mutant strain, TX01ΔhutZ, was constructed. Deletion of hutZ_Ep did not significantly affect bacterial growth in normal medium, in iron-deficient conditions, or in the

SUBMITTER: Shi YJ 

PROVIDER: S-EPMC6775658 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature