CO₂ conversion to methane and biomass in obligate methylotrophic methanogens in marine sediments.

ABSTRACT: Methyl substrates are important compounds for methanogenesis in marine sediments but diversity and carbon utilization by methylotrophic methanogenic archaea have not been clarified. Here, we demonstrate that RNA-stable isotope probing (SIP) requires ¹³C-labeled bicarbonate as co-substrate for identification of methylotrophic methanogens in sediment samples of the Helgoland mud area, North Sea. Using lipid-SIP, we found that methylotrophic methanogens incorporate 60-86% of dissolved inorganic carbon (DIC) into lipids, and thus considerably more than what can be predicted from known metabolic pathways (~40% contribution). In slurry experiments amended with the marine methylotroph Methanococcoides methylutens, up to 12% of methane was produced from CO₂, indicating that C

SUBMITTER: Yin X 

PROVIDER: S-EPMC6775961 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature