Affinity informs environmental cooperation between ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing (Anammox) bacteria.

ABSTRACT: Anaerobic ammonia-oxidizing (Anammox) bacteria (AnAOB) rely on nitrite supplied by ammonia-oxidizing bacteria (AOB) and archaea (AOA). Affinities for ammonia and oxygen play a crucial role in AOA/AOB competition and their association with AnAOB. In this work we measured the affinity constants for ammonia and oxygen (half-saturation; k_m) of two freshwater AOA enrichments, an AOA soil isolate (N. viennensis), and a freshwater AnAOB enrichment. The AOA enrichments had similar kinetics (μ_max ≈ 0.36 d^-1, k_m,NH4 ≈ 0.78 µM, and k_m,O2 ≈ 2.9 µM), whereas N. viennensis had similar k_m values but lower μ_max (0.23 d^-1). In agreement with the current paradigm, these AOA strains showed a higher affinity for ammonia (l

SUBMITTER: Straka LL 

PROVIDER: S-EPMC6775968 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature