Culturing the ubiquitous freshwater actinobacterial acI lineage by supplying a biochemical 'helper' catalase.

ABSTRACT: The actinobacterial acI lineage is among the most successful and ubiquitous freshwater bacterioplankton found on all continents, often representing more than half of all microbial cells in the lacustrine environment and constituting multiple ecotypes. However, stably growing pure cultures of the acI lineage have not been established despite various cultivation efforts based on ecological and genomic studies on the lineage, which is in contrast to the ocean from which abundant microorganisms such as Prochlorococcus, Pelagibacter, and Nitrosopumilus have been isolated. Here, we report the first two pure cultures of the acI lineage successfully maintained by supplementing the growth media with catalase. Catalase was critical for stabilizing the growth of acI strains irrespective of the genomi

SUBMITTER: Kim S 

PROVIDER: S-EPMC6775976 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature