A new widespread subclass of carbonic anhydrase in marine phytoplankton.

ABSTRACT: Most aquatic photoautotrophs depend on CO₂-concentrating mechanisms (CCMs) to maintain productivity at ambient concentrations of CO₂, and carbonic anhydrase (CA) plays a key role in these processes. Here we present different lines of evidence showing that the protein LCIP63, identified in the marine diatom Thalassiosira pseudonana, is a CA. However, sequence analysis showed that it has a low identity with any known CA and therefore belongs to a new subclass that we designate as iota-CA. Moreover, LCIP63 unusually prefers Mn²⁺ to Zn²⁺ as a cofactor, which is potentially of ecological relevance since Mn²⁺ is more abundant than Zn²⁺ in the ocean. LCIP63 is located in the chloroplast and only expressed at low concentrations of C

SUBMITTER: Jensen EL 

PROVIDER: S-EPMC6776030 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature