A mechanism for the activation of the mechanosensitive Piezo1 channel by the small molecule Yoda1.

ABSTRACT: Mechanosensitive Piezo1 and Piezo2 channels transduce various forms of mechanical forces into cellular signals that play vital roles in many important biological processes in vertebrate organisms. Besides mechanical forces, Piezo1 is selectively activated by micromolar concentrations of the small molecule Yoda1 through an unknown mechanism. Here, using a combination of all-atom molecular dynamics simulations, calcium imaging and electrophysiology, we identify an allosteric Yoda1 binding pocket located in the putative mechanosensory domain, approximately 40 Å away from the central pore. Our simulations further indicate that the presence of the agonist correlates with increased tension-induced motions of the Yoda1-bound subunit. Our results suggest a model wherein Yoda1 acts as a molecular w

SUBMITTER: Botello-Smith WM 

PROVIDER: S-EPMC6776524 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature