Endogenous epitope-tagging of <i>Tet1</i>, <i>Tet2</i> and <i>Tet3</i> identifies TET2 as a naive pluripotency marker.

ABSTRACT: Tet1, Tet2, and Tet3 encode DNA demethylases that play critical roles during stem cell differentiation and reprogramming to pluripotency. Although all three genes are transcribed in pluripotent cells, little is known about the expression of the corresponding proteins. Here, we tagged all the endogenous Tet family alleles using CRISPR/Cas9, and characterised TET protein expression in distinct pluripotent cell culture conditions. Whereas TET1 is abundantly expressed in both naïve and primed pluripotent cells, TET2 expression is restricted to the naïve state. Moreover, TET2 is expressed heterogeneously in embryonic stem cells (ESCs) cultured in serum/leukemia inhibitory factor, with expression correlating with naïve pluripotency markers. FACS-sorting of ESCs carryi

SUBMITTER: Pantier R 

PROVIDER: S-EPMC6776666 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature