Supercooling extends preservation time of human livers.

ABSTRACT: The inability to preserve vascular organs beyond several hours contributes to the scarcity of organs for transplantation^1,2. Standard hypothermic preservation at +4 °C (refs. ^1,3) limits liver preservation to less than 12 h. Our group previously showed that supercooled ice-free storage at -6 °C can extend viable preservation of rat livers^4,5 However, scaling supercooling preservation to human organs is intrinsically limited because of volume-dependent stochastic ice formation. Here, we describe an improved supercooling protocol that averts freezing of human livers by minimizing favorable sites of ice nucleation and homogeneous preconditioning with protective agents during machine perfusion. We show that human livers can be stored at -4 °C with supercooling

SUBMITTER: de Vries RJ 

PROVIDER: S-EPMC6776681 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature