Establishment and application of a CRISPR-Cas12a assisted genome-editing system in Zymomonas mobilis.

ABSTRACT:

Background

Efficient and convenient genome-editing toolkits can expedite genomic research and strain improvement for desirable phenotypes. Zymomonas mobilis is a highly efficient ethanol-producing bacterium with a small genome size and desirable industrial characteristics, which makes it a promising chassis for biorefinery and synthetic biology studies. While classical techniques for genetic manipulation are available for Z. mobilis, efficient genetic engineering toolkits enabling rapidly systematic and high-throughput genome editing in Z. mobilis are still lacking.

Results

Using Cas12a (Cpf1) from Francisella novicida, a recombinant strain with inducible cas12a expression for genome editing was constructed in Z. mobilis ZM4, which can be used to mediate RNA-guided DNA cleav

SUBMITTER: Shen W 

PROVIDER: S-EPMC6777028 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature