Silencing of the phytoene desaturase (PDS) gene affects the expression of fruit-ripening genes in tomatoes.

ABSTRACT:

Background

Past research has shown that virus-induced phytoene desaturase (PDS) gene silencing via agroinjection in the attached and detached fruit of tomato plants results in a pale-yellow fruit phenotype. Although the PDS gene is often used as a marker for gene silencing in tomatoes, little is known about the role of PDS in fruit ripening. In this study, we investigated whether the pepper PDS gene silenced endogenous PDS genes in the fruit of two tomato cultivars, Dotaerang Plus and Legend Summer.

Results

We found that the pepper PDS gene successfully silenced endogenous PDS in tomato fruit at a silencing frequency of 100% for both cultivars. A pale-yellow silenced area was observed over virtually the entire surface of indiv

SUBMITTER: Naing AH 

PROVIDER: S-EPMC6777038 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature