Dimethyl Sulfoxide Leads to Decreased Osteogenic Differentiation of Stem Cells Derived from Gingiva via Runx2 and Collagen I Expression.

ABSTRACT:

Objectives

Dimethyl sulfoxide (DMSO) plays various functions, including cellular functions such as cellular growth. The aim of this study was to evaluate the effects of DMSO on the proliferation and osteogenic differentiation of human gingiva-derived stem cells.

Materials and methods

Stem cells derived from gingiva were cultured in the presence of DMSO at concentrations ranging from 0.01 to 10%.

Statistical analysis

We performed a one-way analysis of variance (ANOVA) with post hoc test to determine the differences between the groups using a commercially available program and the level of significance was 0.05.

Results

The cells in the control group showed normal fibroblast morphology. The cells treated with 0.01%, 0.01%, 0.1%, and 1% DMSO were morphologically s

SUBMITTER: Lee H 

PROVIDER: S-EPMC6777167 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature