Characteristics and function of a novel cystatin gene in the pine wood nematode Bursaphelenchus xylophilus.

ABSTRACT: Bursaphelenchus xylophilus is the pathogen that causes pine wilt disease (PWD). The disease has caused significant economic losses and damage to forests. However, the pathogenic mechanism of B. xylophilus remains unclear. Cystatins are involved in various biological processes where they regulate normal proteolysis and also play a role in pathogenicity, but their functions in B. xylophilus are unknown. Therefore, we cloned the full-length cDNA of a cystatin gene of B. xylophilus (Bx-cpi-1) by rapid-amplification of cDNA ends and analyzed its characteristics with bioinformatic methods. In situ mRNA hybridization analyses showed that transcripts of Bx-cpi-1 were abundantly expressed in the reproductive organs of B. xylophilus The express

SUBMITTER: Xue Q 

PROVIDER: S-EPMC6777362 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature