Whole genome sequencing of a novel, dichloromethane-fermenting Peptococcaceae from an enrichment culture.

ABSTRACT: Bacteria capable of dechlorinating the toxic environmental contaminant dichloromethane (DCM, CH₂Cl₂) are of great interest for potential bioremediation applications. A novel, strictly anaerobic, DCM-fermenting bacterium, "DCMF", was enriched from organochlorine-contaminated groundwater near Botany Bay, Australia. The enrichment culture was maintained in minimal, mineral salt medium amended with dichloromethane as the sole energy source. PacBio whole genome SMRT^TM sequencing of DCMF allowed de novo, gap-free assembly despite the presence of cohabiting organisms in the culture. Illumina sequencing reads were utilised to correct minor indels. The single, circularised 6.44 Mb chromosome was annotated with the IMG pipeline and contains 5,773 predicted pro

SUBMITTER: Holland SI 

PROVIDER: S-EPMC6778437 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature