Transformation with Artificial Chromosomes in Oxytricha trifallax and Their Applications.

ABSTRACT: Oxytricha trifallax, like other ciliates, has separate germline and somatic nuclei. The diploid germline genome in the micronucleus is composed of long conventional chromosomes. The macronucleus contains a somatic genome which is naturally fragmented into thousands of kilobase-sized chromosomes. Here, we develop a method to stably incorporate artificial chromosomes into the macronucleus. We report two cases of successful transformation and demonstrate the use of somatic transformation to investigate gene regulation and gene function in Oxytricha We show that the transformed artificial chromosomes are maintained through multiple asexual divisions. Furthermore, they support the transcriptional regulation of the native chromosome from which they were derived and are translated to produce func

SUBMITTER: Clay DM 

PROVIDER: S-EPMC6778790 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature