Involvement of a G Protein Regulatory Circuit in Alternative Oxidase Production in Neurospora crassa.

ABSTRACT: The Neurospora crassa nuclear aod-1 gene encodes an alternative oxidase that functions in mitochondria. The enzyme provides a branch from the standard electron transport chain by transferring electrons directly from ubiquinol to oxygen. In standard laboratory strains, aod-1 is transcribed at very low levels under normal growth conditions. However, if the standard electron transport chain is disrupted, aod-1 mRNA expression is induced and the AOD1 protein is produced. We previously identified a strain of N. crassa, that produces high levels of aod-1 transcript under non-inducing conditions. Here we have crossed this strain to a standard lab strain and determined the genomic sequences of the parents and several progeny. Analysis of the sequence data

SUBMITTER: Bosnjak N 

PROVIDER: S-EPMC6778808 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature