Comparison of different semi-automated cfDNA extraction methods in combination with UMI-based targeted sequencing.

ABSTRACT: The analysis of circulating cell-free DNA (cfDNA) extracted from peripheral blood can serve as a minimally invasive alternative to tumor tissue biopsies in cases with impaired access to tissue. Its clinical utility has been well demonstrated for EGFR T790M testing in lung cancer patients suffering progress after tyrosine kinase inhibitor treatment. At present, highly sensitive unique molecular identifiers (UMI)-based NGS for liquid biopsy testing is less established compared to single gene assays. However, the critical bottleneck are sufficient cfDNA yields, which are essentially required to obtain meaningful test results. We compared four different cfDNA extraction methods (Qiagen, Promega, Thermo and Stratec) using the same plasma samples in order to evaluate their suitability for furthe

SUBMITTER: Streubel A 

PROVIDER: S-EPMC6779285 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature