Standalone interferometry-based calibration of convex lens-induced confinement microscopy with nanoscale accuracy.

ABSTRACT: Strongly confined environments (confined dimensions between 1-100 nm) represent unique challenges and opportunities for understanding and manipulating molecular behavior due to the significant effects of electric double layers, high surface-area to volume ratios, and other phenomena at the nanoscale. Convex Lens-induced Confinement (CLiC) can be used to analyze the dynamics of individual molecules or particles confined in a planar slit geometry with continuously varying gap thickness. We describe an interferometry-based method for precise measurement of the slit pore geometry. Specifically, this approach permitted accurate characterization of separation distances as small as 5 nm, with 1 nm precision, without a priori knowledge or assumptions about the contact geometry, as well as a greatl

SUBMITTER: Morrin GT 

PROVIDER: S-EPMC6779313 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature