Glomerular Function and Structural Integrity Depend on Hyaluronan Synthesis by Glomerular Endothelium.

ABSTRACT:

Background

A glycocalyx envelope consisting of proteoglycans and adhering proteins covers endothelial cells, both the luminal and abluminal surface. We previously demonstrated that short-term loss of integrity of the luminal glycocalyx layer resulted in perturbed glomerular filtration barrier function.

Methods

To explore the role of the glycocalyx layer of the endothelial extracellular matrix in renal function, we generated mice with an endothelium-specific and inducible deletion of hyaluronan synthase 2 (Has2), the enzyme that produces hyaluronan, the main structural component of the endothelial glycocalyx layer. We also investigated the presence of endothelial hyaluronan in human kidney tissue from patients with varying degrees of diabetic nephropathy.

Results

Endot

SUBMITTER: van den Berg BM 

PROVIDER: S-EPMC6779367 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature