Effect of methylation of adenine N⁶ on kink turn structure depends on location.

ABSTRACT: N⁶-methyladenine is the most common covalent modification in cellular RNA species, with demonstrated functional consequences. At the molecular level this methylation could alter local RNA structure, and/or modulate the binding of specific proteins. We have previously shown that trans-Hoogsteen-sugar (sheared) A:G base pairs can be completely disrupted by methylation, and that this occurs in a sub-set ofD/D k-turn structures. In this work we have investigated to what extent sequence context affects the severity with which inclusion of N⁶-methyladenine into different A:G base pairs of a standard k-turn affects RNA folding and L7Ae protein binding. We find that local sequence has a major influence, ranging from complete absence of folding and protein binding to a

SUBMITTER: Ashraf S 

PROVIDER: S-EPMC6779385 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature