Methodology for the establishment of primary porcine vocal fold epithelial cell cultures.

ABSTRACT:

Objective

A current lack of methods for epithelial cell culture significantly hinders our understanding of the role of the epithelial and mucus barriers in vocal fold health and disease. Our first objective was to establish reproducible techniques for the isolation and culture of primary porcine vocal fold epithelial cells. Our second objective was to evaluate the functional significance of cell cultures using an in vitro exposure to an inflammatory cytokine.

Methods

Epithelial cells were isolated from porcine vocal folds and expanded in culture. Characterization of cultures was completed by immunostaining with markers for pan-cytokeratin (epithelial cells), vimentin (stromal cells), von Willebrand factor (endothelial cell), and MUC1 and MUC4 (mucin) glycoproteins. Establish

SUBMITTER: Erickson-DiRenzo E 

PROVIDER: S-EPMC6779414 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature