Alternative fate of glyoxylate during acetate and hexadecane metabolism in Acinetobacter oleivorans DR1.

ABSTRACT: The glyoxylate shunt (GS), involving isocitrate lyase (encoded by aceA) and malate synthase G (encoded by glcB), is known to play important roles under several conditions including oxidative stress, antibiotic defense, or certain carbon source metabolism (acetate and fatty acids). Comparative growth analyses of wild type (WT), aceA, and glcB null-strains revealed that aceA, but not glcB, is essential for cells to grow on either acetate (1%) or hexadecane (1%) in Acinetobacter oleivorans DR1. Interestingly. the aceA knockout strain was able to grow slower in 0.1% acetate than the parent strain. Northern Blot analysis showed that the expression of aceA was dependent on the concentration of acetate or H₂O₂, while glcB was constitutively expressed. Up-regulation of stress

SUBMITTER: Park C 

PROVIDER: S-EPMC6779741 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature