Rapid selection and identification of functional CD8⁺ T cell epitopes from large peptide-coding libraries.

ABSTRACT: Cytotoxic CD8⁺ T cells recognize and eliminate infected or malignant cells that present peptide epitopes derived from intracellularly processed antigens on their surface. However, comprehensive profiling of specific major histocompatibility complex (MHC)-bound peptide epitopes that are naturally processed and capable of eliciting a functional T cell response has been challenging. Here, we report a method for deep and unbiased T cell epitope profiling, using in vitro co-culture of CD8⁺ T cells together with target cells transduced with high-complexity, epitope-encoding minigene libraries. Target cells that are subject to cytotoxic attack from T cells in co-culture are isolated prior to apoptosis by fluorescence-activated cell sorting, and characterized by sequencing th

SUBMITTER: Sharma G 

PROVIDER: S-EPMC6779888 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature