Scaffold-Free Bioprinter Utilizing Layer-By-Layer Printing of Cellular Spheroids.

ABSTRACT: Free from the limitations posed by exogenous scaffolds or extracellular matrix-based materials, scaffold-free engineered tissues have immense clinical potential. Biomaterials may produce adverse responses, interfere with cell-cell interaction, or affect the extracellular matrix integrity of cells. The scaffold-free Kenzan method can generate complex tissues using spheroids on an array of needles but could be inefficient in terms of time, as it moves and places only a single spheroid at a time. We aimed to design and construct a novel scaffold-free bioprinter that can print an entire layer of spheroids at once, effectively reducing the printing time. The bioprinter was designed using computer-aided design software and constructed from machined, 3D printed, and commercially available parts.

SUBMITTER: LaBarge W 

PROVIDER: S-EPMC6780220 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature