Mixture Effects of Diesel Exhaust and Metal Oxide Nanoparticles in Human Lung A549 Cells.

ABSTRACT: Airborne ultrafine particles (UFP) mainly derive from combustion sources (e.g., diesel exhaust particles-DEP), abrasion sources (non-exhaust particles) or from the unintentional release of engineered nanoparticles (e.g., metal oxide nanoparticles-NPs), determining human exposure to UFP mixtures. The aim of the present study was to analyse the combined in vitro effects of DEP and metal oxide NPs (ZnO, CuO) on human lung A549 cells. The mixtures and the relative single NPs (DEP, ZnO, CuO) were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS) and inductively coupled plasma-optic emission spectroscopy (ICP-OES). Cells were exposed for different times (3-72 h) to mixtures of standard DEP at a subcytotoxic concentration and ZnO and CuO at increasing concent

SUBMITTER: Zerboni A 

PROVIDER: S-EPMC6781047 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature