Engineering the oleaginous yeast Yarrowia lipolytica to produce limonene from waste cooking oil.

ABSTRACT:

Background

Limonene is an important biologically active natural product widely used in the food, cosmetic, nutraceutical and pharmaceutical industries. However, the low abundance of limonene in plants renders their isolation from plant sources non-economically viable. Therefore, engineering microbes into microbial factories for producing limonene is fast becoming an attractive alternative approach that can overcome the aforementioned bottleneck to meet the needs of industries and make limonene production more sustainable and environmentally friendly.

Results

In this proof-of-principle study, the oleaginous yeast Yarrowia lipolytica was successfully engineered to produce both d-limonene and l-limonene by introducing the heterologous d-limonene synthase from Citrus l

SUBMITTER: Pang Y 

PROVIDER: S-EPMC6781337 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature