Detection of malaria parasites in dried human blood spots using mid-infrared spectroscopy and logistic regression analysis.

ABSTRACT:

Background

Epidemiological surveys of malaria currently rely on microscopy, polymerase chain reaction assays (PCR) or rapid diagnostic test kits for Plasmodium infections (RDTs). This study investigated whether mid-infrared (MIR) spectroscopy coupled with supervised machine learning could constitute an alternative method for rapid malaria screening, directly from dried human blood spots.

Methods

Filter papers containing dried blood spots (DBS) were obtained from a cross-sectional malaria survey in 12 wards in southeastern Tanzania in 2018/19. The DBS were scanned using attenuated total reflection-Fourier Transform Infrared (ATR-FTIR) spectrometer to obtain high-resolution MIR spectra in the range 4000 cm^-1 to 500 cm^-1. The spectra were cleaned to compen

SUBMITTER: Mwanga EP 

PROVIDER: S-EPMC6781347 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature