The liquid fraction from hydrothermal pretreatment of wheat straw provides lytic polysaccharide monooxygenases with both electrons and H₂O₂ co-substrate.

ABSTRACT:

Background

Enzyme-aided valorization of lignocellulose represents a green and sustainable alternative to the traditional chemical industry. The recently discovered lytic polysaccharide monooxygenases (LPMOs) are important components of the state-of-the art enzyme cocktails for cellulose conversion. Yet, these monocopper enzymes are poorly characterized in terms of their kinetics, as exemplified by the growing evidence for that H₂O₂ may be a more efficient co-substrate for LPMOs than O₂. LPMOs need external electron donors and one key question of relevance for bioprocess development is whether the required reducing power may be provided by the lignocellulosic substrate.

Results

Here, we show that the liquid fraction (LF) resulting from hydroth

SUBMITTER: Kont R 

PROVIDER: S-EPMC6781412 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature