Perlecan regulates pericyte dynamics in the maintenance and repair of the blood-brain barrier.

ABSTRACT: Ischemic stroke causes blood-brain barrier (BBB) breakdown due to significant damage to the integrity of BBB components. Recent studies have highlighted the importance of pericytes in the repair process of BBB functions triggered by PDGFRβ up-regulation. Here, we show that perlecan, a major heparan sulfate proteoglycan of basement membranes, aids in BBB maintenance and repair through pericyte interactions. Using a transient middle cerebral artery occlusion model, we found larger infarct volumes and more BBB leakage in conditional perlecan (Hspg2)-deficient (Hspg2 ^{- / -} -TG) mice than in control mice. Control mice showed increased numbers of pericytes in the ischemic lesion, whereas Hspg2 ^{- /}

SUBMITTER: Nakamura K 

PROVIDER: S-EPMC6781430 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature