RNA-DNA Hybrids Support Recombination-Based Telomere Maintenance in Fission Yeast.

ABSTRACT: A subset of cancers rely on telomerase-independent mechanisms to maintain their chromosome ends. The predominant "alternative lengthening of telomeres" pathway appears dependent on homology-directed repair (HDR) to maintain telomeric DNA. However, the molecular changes needed for cells to productively engage in telomeric HDR are poorly understood. To gain new insights into this transition, we monitored the state of telomeres during serial culture of fission yeast (Schizosaccharomyces pombe) lacking the telomerase recruitment factor Ccq1. Rad52 is loaded onto critically short telomeres shortly after germination despite continued telomere erosion, suggesting that recruitment of recombination factors is not sufficient to maintain telomeres in the absence of telomerase function. Instead

SUBMITTER: Hu Y 

PROVIDER: S-EPMC6781888 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature