Generation of tyrosine hydroxylase-producing neurons from precursors of the embryonic and adult forebrain.

ABSTRACT: We have explored the plastic ability of neuronal precursors to acquire different identities by manipulating their surrounding environment. Specifically, we sought to identify potential signals involved in the specification of forebrain dopaminergic neurons. Here we describe culture conditions under which tyrosine hydroxylase (TH) expression is induced in neuronal precursors, which were derived directly from the embryonic striatum and adult subependyma (SE) of the lateral ventricle or generated from multipotent forebrain stem cells. TH was successfully induced in all of these cell types by 24 hr exposure to basic fibroblast growth factor (FGF2) and glial cell conditioned media (CM). The greatest magnitude of the inductive action was on embryonic striatal precursors. Although FGF2 alone indu

SUBMITTER: Daadi MM 

PROVIDER: S-EPMC6782621 | biostudies-literature | 1999 Jun

REPOSITORIES: biostudies-literature