Cholangiocyte-Derived Exosomal Long Noncoding RNA H19 Promotes Hepatic Stellate Cell Activation and Cholestatic Liver Fibrosis.

ABSTRACT: Activation of hepatic stellate cells (HSCs) represents the primary driving force to promote the progression of chronic cholestatic liver diseases. We previously reported that cholangiocyte-derived exosomal long noncoding RNA-H19 (lncRNA-H19) plays a critical role in promoting cholestatic liver injury. However, it remains unclear whether cholangiocyte-derived lncRNA-H19 regulates HSC activation, which is the major focus of this study. Both bile duct ligation (BDL) and Mdr2 knockout (Mdr2^-/- ) mouse models were used. Wild-type and H19^{maternalΔExon1/+} (H19KO) mice were subjected to BDL. Mdr2^-/- H19^{maternalΔExon1/+} (DKO) mice were generated. Exosomes isolated from cultured mouse and human cholangiocytes or mouse serum were used for in vivo transplant

SUBMITTER: Liu R 

PROVIDER: S-EPMC6783323 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature